Brian Schilder, M.Phil

Department of Genetics & Genomic Sciences

Wildfire

Transcriptomic data from 16k+ individual brain cells (shown as points) after reducing the dimensionality with an autoencoder and UMAP. 5 million tracts are shown interconnecting these cells, where shorter tract length represents greater similarity in their molecular profiles.

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Long Li, PhD

Nash Family Department of Neuroscience

May the 4th be with U

Local infection of excitatory neurons(1st panel blue color) and their input neurons
from hippocampus (green neurons in the other 3 panels, the red ones are projecting neurons to LS).

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Shalaka Wahane, PhD

Nash Family Department of Neuroscience

Hand of Tumors

A glioblastoma progressing through the brain parenchyma utilizing the brain’s vascular structure (not imaged here). The myeloid cells stand guard outside as well as inside the tumour.

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Ni-Ka Ford

Instructional Technology Group

Inside the Neocortex

A prominent neuron found in the neocortex is the pyramidal neuron. It is located in subcortical structures such as the hippocampus and the amygdala. This image strives to capture the intricate beauty and bountifulness of the pyramidal neuron which is responsible for many important cognitive processes. The neurons were generated from data of human pyramidal cells from NeuroMorpho.org and imported into Cinema 4D as accurate 3D models. The image was composited further in photoshop for the finishing touches such as the neural signaling and
atmospheric perspective.

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Anthony Lacagnina, PhD

Nash Family Department of Neuroscience

Fear memory-related neurons

Using an activity-dependent tagging genetic mouse (the ArcCre mouse), neurons that were
active during a contextual fear conditioning session were permanently tagged with a fluorescent reporter. These are hippocampal CA1 neurons that were active during that fearful experience.

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Long Li, PhD

Nash Family Department of Neuroscience

Christmas Tree

Cfos and oxytocin expression pattern in PVH region(unside-down) after social behavior.

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Chrystian Junqueira Alves, PhD

Nash Family Department of Neuroscience

Golden neurons

Plexin-B2-mediated cell-intrinsic stiffness leading to spontaneous neuronal differentiation upon neural induction. β-III tubulin (TUJ1; gold) and DAPI (blue).

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Jillian Beroza; Dolores Hambardzumyan, PhD; Zhihong Chen, PhD

Department of Neurosurgery

Macrophages Clinging Mashup

The image shows tumor-associated macrophages clinging to the blood vessels in glioblastoma. These macrophages derive from either brain microglia (the bushy ones) or circulating monocytes (the rounder ones), and they behave differently in tumor development.

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Jill K Gregory, CMI, FAMI

Instructional Technology Group

CSF Leak through the Cribriform Plate

Cerebrospinal Fluid (CSF) leakage is a potentially fatal condition that may result when a skull base dural defect permits CSF communication between the cranial vault and sinonasal cavities. Flow rate is an important property of CSF leaks that can contribute to surgical decision-making and predispose patients to complications and inferior outcomes. This illustration depicts a dural tear along with cribriform dehiscence that allows CSF to trickle into the sinonasal cavity. Middle and posterior cribriform leaks tend to pool in the sphenoid sinus (SS), which is measurable by the change in fluid level during the course of an imaging session. This provides a non-invasive means to measure CSF flow rate. This illustration appears in the Journal of Neurosurgery, in the article “Correlation of spontaneous and traumatic anterior skull base CSF leak flow rates with fluid pattern on early, delayed, and subtraction volumetric extended echo train T2-weighted MRI” by Rutland et al.

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James Vicari, (Kellner Lab)

Department of Neurosurgery

The Deadly Wanderer

This image is of a human acute ischemic stroke clot using MSB trichrome staining to
visualize RBC, WBC, platelet and fibrin composition in stroke clots.

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Holly Oemke Madarash

Department of Neurosurgery

Escape

Recolored 3D visualization of a patient’s brain anatomy.

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Genoveva Uzunova, MD, PhD

Department of Pathology

Glioblastoma cells braid

Phase contrast photomicrograph of primary human glioblastoma cells dissociated from a
tumor obtained at surgery and grown for 2 weeks in vitro in cell culture. The image is taken with the new Leica PAULA imaging system.

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Estrella Lopez-Gordo, PhD

Department of Cardiology

When science meets art in a mouse brain

This image illustrates the brain of a healthy adult CMAH knock-out female mouse. The diversity and dynamism of structures in the depicted brain shows us how science can both educate us and inspire us to find beauty beyond the obvious. Maakia amurensis II (MALII) staining in blue and nuclei in pink. Magnification 5x.

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Eric Parise, PhD

Nash Family Department of Neuroscience

Virally targeted astrocytes within the nucleus accumbens

Astrocytes (star-shaped support cells of the brain that are influenced by stress and
depression) labeled with a virus expressing green fluorescent protein (GFP) in a deep brain reward center of mice. Astrocytes are in green; Neurons are in red; and Cell nuclei, are in blue.

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Devina Ung, PhD, Andrea Boitnott and Danielle Mendonca

Department of Psychiatry

Itsy Brainy Spider

Mouse cortical neuron expressing mCherry (red) immunostained for dendritic marker
MAP2 (magenta), synaptic marker PSD95 (green), and axon marker Tau in blue.

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Anirudh Sattiraju, PhD

Nash Family Department of Neuroscience

Scavengers

Macrophages (red) inside a necrotic region of glioblastoma with tumor cells within
surrounding peri-necrotic region expressing a genetic reporter for hypoxia (green).

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Amy Zhong

Instructional Technology Group

How to Maximize Your Productivity

There are countless articles listing helpful tricks to maximize productivity. In this Rube
Goldberg inspired illustration I’ve depicted an artist putting these tricks into practice. Attempting to start her day off early (tip #1), the artist sets her phone alarm (A) to go off at 4AM. Annoyed by the noise, the artist (B) picks up the baseball bat (C) and smashes the phone not only eliminating the distractions (tip #2) of social media, but also completing her exercise (tip #3) for the day. Whilst picking up the baseball bat, the string attached is lifted,
thus lowering the thinking cap (D) onto her head allowing her to focus (tip #4). Now that the artist’s stress level has been lowered and has got her blood flow pumping, she gets out of bed and reaches for her robe (E), upsetting the coatrack (F), frightening the chicken (G) atop to hatch an egg (H), or idea. The egg is accidentally kicked into a glass of orange juice (I) which is high in Vitamin D allowing it to give off natural sunlight (tip #5) thereby keeping the artist’s circadian clock in check. A goldfish (J) that just happens to be swimming in this glass of orange juice is irritated by the floating egg. The freshness of an egg can be tested by placing it in a bowl of water. If the egg is buoyant, or stays afloat, it means air has entered the shell and the egg is no longer fresh. The fish slaps the egg out from within the \glass hitting the canvas (K), thereby creating the next Unicorn of the Modern Art World.
The remaining eggs, which are high in protein, provide valuable nutrients as part of a balanced diet (tip #6).

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Alexander Smith, PhD

Nash Family Department of Neuroscience

Reward on the Brain

This is an image of a cleared mouse brain, with the green channel representing
autofluorescence, primarily in white matter axon tracts. The red channel is immunofluorescence indicating c-Fos immunoreactivity, showing cells that were activated following a novel reward learning task.

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Esther Cheng

Nash Family Department of Neuroscience

Cerebral Brain Organoid

Immunocytochemistry of a cerebral brain organoid cultured from induced pluripotent stem cells.

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Jean-Vianney Haure-Mirande, PhD

Department of Neurology

Astrocytes around Amyloid plaque

GFAP positive astrocytes (green) recruited around Aß plaque (red) in the APP/PS1 mouse
model of Alzheimer’s pathology

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Mickael Audrain, PhD

Department of Neurology

The U.S. Border

Primary microglia and astrocytes.

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Zhuhao Wu, PhD

Department of Cell, Developmental and Regenerative Biology

Cerebellar Purkinje Cell Projections in 3D

This picture is a volume imaging of sparsely labeled Purkinje cells in an adult mouse brain to reveal their full morphology in 3D. Color indicates the image depth (bottom right scale). The whole tissue was rendered optically transparent with iDISCO techniques and imaged with light-sheet fluorescent microscope to visualize individual Purkinje neurons for their entire long and curved projections from the cell bodies near the surface of the cerebellum to the deep cerebellar nuclei.

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